9Q7V

One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	108
Detector technology	PIXEL
Collection date	2017-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	49.007, 63.256, 167.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.402 - 2.356
R-factor	0.1924
Rwork	0.187
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.255
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.356	5.080	2.360
Rmerge	0.090	0.074	0.322
Rmeas	0.097	0.081	0.361
Rpim	0.037	0.032	0.160
Number of reflections	22176	2393	2083
<I/σ(I)>	10.6
Completeness [%]	99.4	98.7	95.8
Redundancy	6.5	6.2	4.8
CC(1/2)		0.992	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM HEPES pH 7.0, 200mM ammonium sulfate, 32.5% PEG 3350