9Q6A

Crystal structure of octameric COMMD-like protein MBK5214510 from Flavobacteriaceae bacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	I 2 2 2
Unit cell lengths	91.343, 96.013, 115.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.670 - 2.580
R-factor	0.2452
Rwork	0.239
R-free	0.29230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.470
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.700	2.660
High resolution limit [Å]	2.580	2.580
Rpim	0.022	0.146
Number of reflections	16103	1127
<I/σ(I)>	14.8	2.8
Completeness [%]	98.7
Redundancy	6.6
CC(1/2)	0.999	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% DMSO, 0.1 M sodium chloride, 25% PE5/4