9Q68

Anti-HPA-1a Fab D204 and integrin beta 3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.68880
Spacegroup name	P 21 21 21
Unit cell lengths	93.951, 94.404, 126.501
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.520 - 2.400
R-factor	0.1917
Rwork	0.188
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.922
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.400	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	44723	4633
<I/σ(I)>	8.2
Completeness [%]	99.9
Redundancy	13.6
CC(1/2)	0.994	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	SODIUM CITRATE, PEG4000, 2-PROPANOL