9Q5K
[23-7B C|A] 23 bp tensegrity triangle that propagates via blunt-end stacking with C stacking on A at the interface
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.007430 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 183.136, 183.136, 183.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.750 - 9.960 |
| R-factor | 0.29 |
| Rwork | 0.282 |
| R-free | 0.35480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.992 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 81.901 | 81.901 | 11.871 |
| High resolution limit [Å] | 9.960 | 15.209 | 9.961 |
| Number of reflections | 646 | 214 | 214 |
| <I/σ(I)> | 11.3 | ||
| Completeness [%] | 93.1 | ||
| Redundancy | 65.3 | ||
| CC(1/2) | 0.997 | 0.997 | 0.511 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |
