9Q5H

anti-HPA-1a Fab B2G1 CONFORMATION 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97872
Spacegroup name	P 41 21 2
Unit cell lengths	68.020, 68.020, 232.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.280 - 2.000
R-factor	0.1929
Rwork	0.191
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.859
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.390	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	36795	2154
<I/σ(I)>	20.9
Completeness [%]	97.0
Redundancy	20.2
CC(1/2)	0.999	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	Morpheus B6, Halogens, Buffer system 2, Precipitant Mix 2.