9Q4L
anti-HPA-1a Fab 26.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.980, 98.480, 69.810 |
| Unit cell angles | 90.00, 98.86, 90.00 |
Refinement procedure
| Resolution | 45.350 - 2.600 |
| R-factor | 0.1831 |
| Rwork | 0.180 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.004 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.900 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 26812 | 3257 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6 | |
| CC(1/2) | 0.981 | 0.316 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292.15 | PEG 8000, MES/sodium hydroxide, calcium acetate |
