9Q3W

[22-7B C|A] 22 bp tensegrity triangle that propagates via blunt-end stacking with C stacking on A at the interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92021
Spacegroup name	I 41 3 2
Unit cell lengths	178.630, 178.630, 178.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.930 - 6.080
R-factor	0.2028
Rwork	0.202
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.884
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.930	72.925	6.540
High resolution limit [Å]	6.080	11.882	6.084
Number of reflections	1195	199	199
<I/σ(I)>	16.8
Completeness [%]	91.2
Redundancy	25.3
CC(1/2)	0.999	0.999	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate