9Q2Y

Phosphodiesterase from Burkholderia phage BCSR5 in the open lid conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-29
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	72.513, 74.800, 76.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.080 - 2.030
R-factor	0.2283
Rwork	0.227
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.814
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.080	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.166
Number of reflections	27651	2023
<I/σ(I)>	12
Completeness [%]	100.0
Redundancy	8.8
CC(1/2)	0.997	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, 20% w/v PEG8000