9Q2G

Phosphodiesterase from Burkholderia phage BCSR5 in the closed lid conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 43
Unit cell lengths	64.968, 64.968, 50.426
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.830 - 2.000
R-factor	0.1525
Rwork	0.148
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.970	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.062	0.145
Number of reflections	13622	770
<I/σ(I)>	18.4	7.5
Completeness [%]	95.1	73.8
Redundancy	5.7
CC(1/2)	0.998	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	294	0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, 30% PEG4000