9Q0H

One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	108
Detector technology	PIXEL
Collection date	2017-10-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999993
Spacegroup name	P 21 21 21
Unit cell lengths	39.278, 75.539, 167.234
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.854 - 2.200
R-factor	0.2022
Rwork	0.198
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.913
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.200	4.850	2.250
Rmerge	0.090	0.058	0.998
Rmeas	0.097	0.063	1.079
Rpim	0.036	0.023	0.402
Number of reflections	25984	2642	2380
<I/σ(I)>	9
Completeness [%]	99.7	99.9	99.5
Redundancy	7.6	7.4	7
CC(1/2)		0.998	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM HEPES, pH 7.5, 200 mM ammonium sulfate, 25% PEG3350