9Q0C
TEM-1 WT in complex with BLIP E73W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2019-03-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.960, 105.220, 82.070 |
| Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
| Resolution | 38.040 - 1.780 |
| R-factor | 0.1781 |
| Rwork | 0.176 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.832 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.040 | 1.800 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.053 | |
| Number of reflections | 75080 | 2530 |
| <I/σ(I)> | 8.92 | 2.41 |
| Completeness [%] | 94.8 | 88 |
| Redundancy | 2.1 | 2 |
| CC(1/2) | 0.976 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M magnesium chloride, 0.1 M sodium acetate pH 4.6, 25% (w/v) PEG 400 |
