9Q0A

CTX-M-14 WT in complex with BLIP E73W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	298
Detector technology	CCD
Collection date	2019-03-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1
Spacegroup name	P 2 21 21
Unit cell lengths	43.170, 79.890, 118.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.480 - 1.650
R-factor	0.1874
Rwork	0.186
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.842
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.480	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	49766	2744
<I/σ(I)>	12.28
Completeness [%]	99.5
Redundancy	4.2
CC(1/2)	0.992	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M calcium chloride, 0.1 M sodium acetate pH 5, 20% (w/v) PEG 6000