9Q07
Structure-based design of a nutrient acquisition protein enhances neutralizing antibodies and protection for the development of a gonococcal vaccine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2017-05-30 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.668, 60.182, 183.284 |
| Unit cell angles | 90.00, 110.71, 90.00 |
Refinement procedure
| Resolution | 49.560 - 2.600 |
| R-factor | 0.1889 |
| Rwork | 0.188 |
| R-free | 0.21610 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.609 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.560 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.590 |
| Number of reflections | 63133 | 2336 |
| <I/σ(I)> | 24.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.7 | |
| CC(1/2) | 0.500 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Sodium cacodylate pH 6.5, 1 M sodium citrate, 15 % glycerol |
