9PZE

Crystal structure of Fe/2-OG dependent dioxygenase MysH with docked C-terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03321
Spacegroup name	P 21 21 2
Unit cell lengths	88.505, 142.701, 44.612
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.900 - 1.680
R-factor	0.1825
Rwork	0.181
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.21.1_5286)
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.900	1.700
High resolution limit [Å]	1.679	1.680
Number of reflections	124593	54
<I/σ(I)>	7.84	2.18
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.996	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	277.15	20% PEG 3000, citrate pH 5.5