9PZ5

Native anti-NANP Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-13
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	45.657, 72.785, 138.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 2.390
R-factor	0.2
Rwork	0.194
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.843
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.670	2.475
High resolution limit [Å]	2.390	2.390
Number of reflections	35347	1840
<I/σ(I)>	18.34
Completeness [%]	99.7
Redundancy	13.3
CC(1/2)	0.999	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl, pH 7.0, 20% (w/v) PEG 1000