9PZ3

Crystal structure of HIV Apex targeting neutralizing antibody Q12BBM-069

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91972
Spacegroup name	P 21 21 21
Unit cell lengths	56.419, 72.502, 175.215
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.497 - 1.790
R-factor	0.2125
Rwork	0.211
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.323
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.497	1.818
High resolution limit [Å]	1.787	1.787
Number of reflections	68718	3389
<I/σ(I)>	13.9
Completeness [%]	99.6
Redundancy	13.8
CC(1/2)	0.999	0.392

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.26 M NH4-sulfate, 0.1 M cacodylate pH 6.5