9PZ2

Crystal structure of HIV Apex neutralizing Fab Q12QBM-007

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91972
Spacegroup name	P 21 21 21
Unit cell lengths	72.866, 89.150, 89.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.136 - 1.544
Rwork	0.190
R-free	0.20830
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.136	1.571
High resolution limit [Å]	1.544	1.544
Rmerge		1.318
Number of reflections	84833	3540
<I/σ(I)>	13	1
Completeness [%]	98.2
Redundancy	10.3
CC(1/2)	0.999	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50% PEG-200, 0.1M citrate pH 5.5, final pH 6.9