9PYY

Crystal structure of HIV Apex Fab Q10M_055

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91972
Spacegroup name	P 21 21 21
Unit cell lengths	50.689, 74.496, 135.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.650 - 1.630
R-factor	0.2178
Rwork	0.216
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.209
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.735	1.657
High resolution limit [Å]	1.629	1.629
Number of reflections	65019	3206
<I/σ(I)>	15.5	1
Completeness [%]	100.0
Redundancy	13.6
CC(1/2)	0.999	0.361

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100.000 mM HEPES free acid/Sodium hydroxide pH: 7.5, 10.000 %v/v Ethylene glycol 20.000 %w/v PEG 8000