9PYR
Importin alpha 2 in complex with Haliotid herpesvirus 1 large tegument protein NLS region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.537, 90.847, 100.948 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.710 - 2.600 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.848 |
| Data scaling software | Aimless (0.7.13) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.710 | 2.710 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.041 | 0.328 |
| Number of reflections | 22759 | 15183 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 0.6M sodium citrate, 0.1M HEPES pH 7.0, and 10mM DTT |
