9PYJ

Crystal Structure of ZETA_2-Apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97920
Spacegroup name	P 1 21 1
Unit cell lengths	42.210, 94.865, 50.375
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.426 - 3.490
Rwork	0.225
R-free	0.28290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.111
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.82))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.860	3.820
High resolution limit [Å]	3.490	3.490
Rmerge	0.335	0.934
Rmeas	0.405
Rpim	0.155	0.442
Number of reflections	5069	1222
<I/σ(I)>	3.2	1.8
Completeness [%]	99.0
Redundancy	6.7	6.7
CC(1/2)	0.963	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M MgCl2, 0.1M Tris pH 8.5, 30% PEG4000