9PYA

Menin mutant - G331D in complex with Ziftomenib

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	48.917, 80.050, 124.333
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.602 - 1.450
Rwork	0.154
R-free	0.17970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.480
High resolution limit [Å]	1.450	1.450
Rmerge	0.080	0.558
Number of reflections	86815	4260
<I/σ(I)>	40.61	2.36
Completeness [%]	99.9
Redundancy	10.7	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.2 M lithium sulfate, 0.1 M HEPES, pH 7.5, 25% (w/v) PEG-3,350