9PY1

PRMT5 Bound to Compound 8 and MTA

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03769
Spacegroup name	I 2 2 2
Unit cell lengths	101.960, 136.338, 178.513
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.270 - 3.040
R-factor	0.2176
Rwork	0.215
R-free	0.25980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.484
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.270	3.180
High resolution limit [Å]	3.040	3.040
Rmerge	0.307	1.812
Rmeas	0.333	1.963
Rpim	0.127	0.750
Number of reflections	24347	2976
<I/σ(I)>	5.7	0.94
Completeness [%]	99.6
Redundancy	6.7
CC(1/2)	0.978	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	11-22% PEG 3350, 200 mM ammonium sulfate