9PXZ

PRMT5 Bound to Compound 6 and MTA

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03580
Spacegroup name	I 2 2 2
Unit cell lengths	103.607, 138.003, 177.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.770 - 2.470
R-factor	0.2262
Rwork	0.224
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.538
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.770	2.520
High resolution limit [Å]	2.470	2.470
Rmerge	0.177	2.362
Rmeas	0.192	2.564
Rpim	0.074	0.989
Number of reflections	46007	2794
<I/σ(I)>	8.28	0.74
Completeness [%]	99.6
Redundancy	6.7
CC(1/2)	0.994	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	11-22% PEG 3350, 200 mM ammonium sulfate