9PWN
Crystal structure of Fabs 7411 in complex with TREM2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000070 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.650, 71.760, 105.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.960 - 1.800 |
| R-factor | 0.17974 |
| Rwork | 0.178 |
| R-free | 0.21786 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.299 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 174028 | 10755 |
| <I/σ(I)> | 8.52 | |
| Completeness [%] | 99.8 | 94.7 |
| Redundancy | 5.2 | 2.7 |
| CC(1/2) | 0.998 | 0.565 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 11% w/v PEG 8000, 240 mM ammonium sulfate, 100 mM MES |
