9PVW

RNA polymerase II elongation complex with dA at +1 site, 8-oxo-GMP added in Syn-conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-06
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	C 1 2 1
Unit cell lengths	166.046, 223.243, 192.764
Unit cell angles	90.00, 100.48, 90.00

Refinement procedure

Resolution	49.370 - 3.560
R-factor	0.248
Rwork	0.247
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.757
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.370	3.630
High resolution limit [Å]	3.560	3.560
Rmerge	0.537	3.967
Rmeas	0.581	4.272
Rpim	0.218	1.577
Total number of observations	576376	32805
Number of reflections	82439	4518
<I/σ(I)>	4.7	0.5
Completeness [%]	99.9
Redundancy	7	7.3
CC(1/2)	0.978	0.220

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000