9PVV
RNA polymerase II elongation complex with dC at +1 site, 8-oxo-GTP bound in A-site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.518, 222.702, 191.185 |
| Unit cell angles | 90.00, 97.82, 90.00 |
Refinement procedure
| Resolution | 38.520 - 3.480 |
| R-factor | 0.2188 |
| Rwork | 0.218 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.682 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.810 | 3.550 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 0.298 | 2.215 |
| Rmeas | 0.321 | 2.380 |
| Rpim | 0.118 | 0.864 |
| Total number of observations | 605364 | 34168 |
| Number of reflections | 83763 | 4573 |
| <I/σ(I)> | 5.5 | 1.4 |
| Completeness [%] | 99.1 | |
| Redundancy | 7.2 | 7.5 |
| CC(1/2) | 0.993 | 0.709 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
