9PVE

HRAS complex with UM0152533 compound

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	126.970, 36.850, 142.030
Unit cell angles	90.00, 93.03, 90.00

Refinement procedure

Resolution	47.280 - 2.000
R-factor	0.2036
Rwork	0.202
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.872
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.280	2.010
High resolution limit [Å]	1.900	1.900
Rmeas	0.106	0.776
Number of reflections	44960	4441
<I/σ(I)>	10.4	2.26
Completeness [%]	99.5	99.64
Redundancy	3.1	2.5
CC(1/2)	0.998	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293.15	0.1 M Sodium citrate, 16 % PEG 3,350