9PUT

HRAS complex with UM0152535 compound

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	127.850, 36.940, 69.720
Unit cell angles	90.00, 96.96, 90.00

Refinement procedure

Resolution	35.470 - 2.000
R-factor	0.2078
Rwork	0.206
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.160
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.470	1.760
High resolution limit [Å]	1.650	1.650
Rmeas	0.046	1.731
Number of reflections	22189	2211
<I/σ(I)>	16.9	1.05
Completeness [%]	99.6	99.1
Redundancy	6.2	1.25
CC(1/2)	0.999	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	293.15	0.1 M Bis-Tris, 0.2 M NaCl2, 21 % PEG 3,350