9PUE
Structure of Alpha Appendage of AP2 bound to the FxDxF motif derived of CCDC32
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS 3.0 MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-26 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.807, 72.465, 42.053 |
| Unit cell angles | 90.00, 99.09, 90.00 |
Refinement procedure
| Resolution | 20.880 - 1.910 |
| R-factor | 0.1872 |
| Rwork | 0.182 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.805 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.880 | 1.970 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.186 | 0.862 |
| Rpim | 0.063 | 0.301 |
| Number of reflections | 36869 | 1771 |
| <I/σ(I)> | 10.1 | 2.4 |
| Completeness [%] | 99.6 | 95.9 |
| Redundancy | 9.5 | |
| CC(1/2) | 0.995 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 40% isopropanol, 100 mM imidazole pH 6.5, 15% PEG 8000 |
