9PTJ

Crystal structure of C. elegans PUF-3 in complex with RNA II-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	160.774, 160.774, 99.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.940 - 2.800
R-factor	0.2484
Rwork	0.247
R-free	0.27070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.231
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.950	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.167
Rmeas	0.182
Rpim	0.071	0.925
Number of reflections	36703	3645
<I/σ(I)>	8	1.2
Completeness [%]	99.7
Redundancy	6.47
CC(1/2)	0.997	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	3.0 ~ 3.5 M sodium formate, 0.1 M bis-tris propane pH 5.0 ~ 6.0