9PTE

Stabilized Y188N variant of the internal UBA Domain of HHR23A in the P1 21 1 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-16
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	30.714, 40.497, 31.703
Unit cell angles	90.00, 90.38, 90.00

Refinement procedure

Resolution	31.700 - 1.150
R-factor	0.1534
Rwork	0.153
R-free	0.16330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.931
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.700	1.190
High resolution limit [Å]	1.150	1.150
Rmerge	0.049	0.107
Rmeas	0.062	0.150
Rpim	0.037	0.105
Number of reflections	57209	796
<I/σ(I)>	12.33	4.66
Completeness [%]	96.0	74.92
Redundancy	2.1	1.4
CC(1/2)	0.994	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M ammonium sulfate, 0.1 M Bis-Tris (pH 5.5), 25% w/v PEG3350