9PT3
Q108K:K40L:T51V:T53S:R58W:Y19W:L117E mutant of hCRBPII bound to fluorophore TD-1V-9
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.405, 66.742, 64.217 |
| Unit cell angles | 89.73, 90.28, 90.10 |
Refinement procedure
| Resolution | 33.370 - 1.790 |
| R-factor | 0.2201 |
| Rwork | 0.218 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.883 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.370 | 1.840 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.065 | 0.614 |
| Number of reflections | 44915 | 4209 |
| <I/σ(I)> | 24.07 | |
| Completeness [%] | 96.5 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG4000, ammonium acetate, 100 mM sodium acetate, pH 4.0 - 4.8 |
