9PSY

Q108K:K40L:T51V:T53S:R58W:Y19W:L117E mutant of hCRBPII bound to fluorophore TD-1V-1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	29.297, 66.840, 64.068
Unit cell angles	90.00, 90.75, 90.00

Refinement procedure

Resolution	33.420 - 1.620
R-factor	0.2223
Rwork	0.221
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.869
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.420	1.680
High resolution limit [Å]	1.620	1.620
Rmerge	0.058	0.679
Number of reflections	30514	2883
<I/σ(I)>	37.8
Completeness [%]	96.5
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG4000, ammonium acetate, 100 mM sodium acetate, pH 4.0 - 4.8