9PSR

Co-crystal structure of BAF60A (SMARCD1) bound to PPSC-002

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-07
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.95373
Spacegroup name	C 1 2 1
Unit cell lengths	82.320, 67.580, 81.075
Unit cell angles	90.00, 123.40, 90.00

Refinement procedure

Resolution	49.300 - 2.210
R-factor	0.23172
Rwork	0.229
R-free	0.28452
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.174
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	2.280
High resolution limit [Å]	2.210	2.210
Number of reflections	18758	1616
<I/σ(I)>	16.2
Completeness [%]	99.9
Redundancy	6.8
CC(1/2)	0.999	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2M Potassium fluoride pH 7.3, 20% w/v PEG 3350