9PSQ
Co-crystal structure of BAF60A (SMARCD1) bound to PPSC-001
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.180, 70.200, 76.300 |
| Unit cell angles | 90.00, 118.92, 90.00 |
Refinement procedure
| Resolution | 49.370 - 2.310 |
| R-factor | 0.21948 |
| Rwork | 0.218 |
| R-free | 0.25757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.266 |
| Data reduction software | DIALS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.390 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Number of reflections | 16082 | 1579 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.997 | 0.948 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2M Ammonium formate pH 6.6, 20% w/v PEG3350 |
