9PSJ

Crystal structure of BAF60A (SMARCD1) apo containing partial coiled coil and the YEATS-like domain with topologically-linked SWIB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	C 1 2 1
Unit cell lengths	82.555, 69.346, 77.958
Unit cell angles	90.00, 120.47, 90.00

Refinement procedure

Resolution	35.580 - 2.090
R-factor	0.21389
Rwork	0.211
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.498
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.600	2.140
High resolution limit [Å]	2.090	2.090
Number of reflections	22251	1594
<I/σ(I)>	10.7
Completeness [%]	98.8
Redundancy	3.7
CC(1/2)	0.994	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	20% w/v PEG3350, 0.2 M NH4NO3