9PSC

Crystal structure of C. elegans PUF-3 in complex with pos-1 RNA mut1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	161.152, 161.152, 100.013
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.180 - 2.400
R-factor	0.2214
Rwork	0.220
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.142
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.080	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.147
Rmeas	0.159
Rpim	0.060	0.721
Number of reflections	58529	5758
<I/σ(I)>	9.2	1.5
Completeness [%]	99.8	99.64
Redundancy	6.91	6.71
CC(1/2)	0.997	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	3.0 ~ 3.5 M sodium formate, 0.1 M bis-tris propane pH 5.0 ~ 6.0