9PSA

Crystal structure of C. elegans PUF-3 in complex with RNA III-9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 62 2 2
Unit cell lengths	143.377, 143.377, 120.042
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.350 - 2.860
R-factor	0.2271
Rwork	0.225
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.997
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.350	3.010
High resolution limit [Å]	2.860	2.860
Rmerge	0.175	2.276
Rmeas	0.184	2.385
Rpim	0.055	0.705
Total number of observations	187402	27606
Number of reflections	17375	2475
<I/σ(I)>	10.2	1.3
Completeness [%]	99.8
Redundancy	10.8	11.2
CC(1/2)	0.998	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.05 M Ammonium sulfate, 0.05 M Bis-Tris pH 5.7~6.5, 26% (v/v) Pentaerythritol ethoxylate (15/4 EO/OH)