9PS9
High-resolution crystal structure of Vibrio cholerae NFeoB in the apo form in orthorhombic space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97935 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.483, 101.106, 301.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.530 - 1.850 |
| R-factor | 0.17761 |
| Rwork | 0.176 |
| R-free | 0.20728 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.428 |
| Data reduction software | AutoProcess |
| Data scaling software | AutoProcess |
| Phasing software | AutoProcess |
| Refinement software | REFMAC (4.4.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.530 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 125249 | 6208 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 14 | |
| CC(1/2) | 0.997 | 0.373 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.4 M lithium sulfate, 0.1 M HEPES sodium, pH 7.2, 2 % (v/v) trifluoroethane |
