9PQW

Crystal structure of C. elegans PUF-3 in complex with spn-4 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 62 2 2
Unit cell lengths	140.837, 140.837, 119.408
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.540 - 2.300
R-factor	0.1962
Rwork	0.194
R-free	0.23220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.729
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.110	0.967
Number of reflections	31545	1534
<I/σ(I)>	25	3.1
Completeness [%]	100.0
Redundancy	15.8
CC(1/2)	0.882	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.05 M Ammonium sulfate, 0.05 M Bis-Tris pH 5.7~6.5, 26% (v/v) Pentaerythritol ethoxylate (15/4 EO/OH)