9PQU

MIF Y98C homotrimeric mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	93
Detector technology	PIXEL
Collection date	2022-03-18
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5417
Spacegroup name	P 21 21 21
Unit cell lengths	67.632, 67.804, 87.439
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.880 - 1.700
R-factor	0.1649
Rwork	0.163
R-free	0.20230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.798
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.18_3845: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	147.880	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.024	0.065
Number of reflections	44906	2330
<I/σ(I)>	32.3	3.4
Completeness [%]	100.0
Redundancy	1.6
CC(1/2)	0.999	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2 M ammonium sulfate, 3% 2-propanol, 0.1 M Tris-HCl, pH 7.5