9PQL

Crystal structure of maltose binding protein (Apo), mutant Trp62 to 4-Fluorotryptophan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953647986
Spacegroup name	P 1 21 1
Unit cell lengths	43.841, 64.621, 57.773
Unit cell angles	90.00, 101.42, 90.00

Refinement procedure

Resolution	42.970 - 1.370
Rwork	0.161
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.970	1.390
High resolution limit [Å]	1.370	1.370
Number of reflections	63791	3182
<I/σ(I)>	10.8
Completeness [%]	96.3
Redundancy	7.2
CC(1/2)	0.999	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	30% v/v PEG400, 100 mM sodium acetate, pH 4.6, 100 mM cadmium chloride