9PQK

Crystal structure of maltose binding protein (Apo), mutant Trp10 to 4-Fluorotryptophan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953724371
Spacegroup name	P 1 21 1
Unit cell lengths	43.956, 64.974, 57.633
Unit cell angles	90.00, 100.97, 90.00

Refinement procedure

Resolution	42.670 - 1.870
R-factor	0.2084
Rwork	0.205
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.105
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5058: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.150	1.910
High resolution limit [Å]	1.870	1.870
Number of reflections	26310	1695
<I/σ(I)>	5.9
Completeness [%]	99.6
Redundancy	6.7
CC(1/2)	0.994	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298.5	30% v/v PEG400, 100 mM sodium acetate, pH 4.6, 100 mM cadmium chloride