Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.433, 136.936, 38.653 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.460 - 1.240 |
| R-factor | 0.13513 |
| Rwork | 0.133 |
| R-free | 0.17090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.503 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC ((Version 1.1.7)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.460 | 1.340 |
| High resolution limit [Å] | 1.240 | 1.244 |
| Rmerge | 0.039 | 1.164 |
| Rmeas | 0.037 | |
| Number of reflections | 119188 | 5959 |
| <I/σ(I)> | 20.6 | 1.4 |
| Completeness [%] | 80.0 | 20.3 |
| Redundancy | 8.1 | 7.3 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 285 | 19% (w/v) PEG3350, 0.17M Magnesium formate |
