9PNJ

Crystal structure of the GH29-like fucosidase FucWf4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97947
Spacegroup name	P 21 21 21
Unit cell lengths	85.973, 99.332, 124.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.450 - 2.200
R-factor	0.1916
Rwork	0.190
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.860
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.450	2.260
High resolution limit [Å]	2.200	2.200
Rmerge		1.000
Number of reflections	54172	4341
<I/σ(I)>	10.1
Completeness [%]	98.7
Redundancy	13.7
CC(1/2)	0.997	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 8000, 0.2 M MgCl2, 0.1 M Tris pH 8.5