9PMM

Crystal structure of C. elegans PUF-3 in complex with RNA I-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	158.328, 158.328, 40.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.840 - 2.150
R-factor	0.1804
Rwork	0.178
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.821
Data reduction software	HKL-2000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.114	0.822
Rmeas	0.125	0.910
Rpim	0.051
Number of reflections	32103	1556
<I/σ(I)>	15.1	2
Completeness [%]	99.7
Redundancy	5.9
CC(1/2)	0.990	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.2 M sodium malonate, pH 5.0, 20% (w/v) PGE 3350