9PMH

[22-7B G|A] 22 bp tensegrity triangle that propagates via blunt-end stacking with G stacking on A at the interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00743
Spacegroup name	I 41 3 2
Unit cell lengths	178.017, 178.017, 178.017
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.960 - 6.200
R-factor	0.2064
Rwork	0.206
R-free	0.20890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.787
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.675	72.675	6.797
High resolution limit [Å]	6.200	12.169	6.202
Number of reflections	1140	190	184
<I/σ(I)>	17.9
Completeness [%]	92.5
Redundancy	49.6
CC(1/2)	1.000	1.000	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate