9PMG

[22-7B G|T] 22 bp tensegrity triangle that propagates via blunt-end stacking with G stacking on T at the interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.007430
Spacegroup name	P 63
Unit cell lengths	133.133, 133.133, 62.784
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.430 - 5.680
R-factor	0.1247
Rwork	0.121
R-free	0.16370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.107
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.567	66.566	6.305
High resolution limit [Å]	5.680	12.552	5.680
Number of reflections	1570	195	195
<I/σ(I)>	15.6
Completeness [%]	88.9
Redundancy	19.5
CC(1/2)	1.000	1.000	0.494

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate