9PLZ

[20-7B G|A] 20 bp tensegrity triangle that propagates via blunt-end stacking with G stacking on A at the interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00743
Spacegroup name	I 41 3 2
Unit cell lengths	165.550, 165.550, 165.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.470 - 5.940
R-factor	0.1299
Rwork	0.127
R-free	0.16730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.500
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.586	67.586	6.649
High resolution limit [Å]	5.940	10.916	5.943
Number of reflections	1032	210	207
<I/σ(I)>	33.7
Completeness [%]	91.4
Redundancy	70.9
CC(1/2)	1.000	1.000	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate