9PLX
The structure of O-glycopeptidase BcM60K from Bacteroides caccae in complex with a core 2 glycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-14 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.924, 64.867, 93.579 |
| Unit cell angles | 90.00, 100.81, 90.00 |
Refinement procedure
| Resolution | 19.580 - 2.490 |
| R-factor | 0.2014 |
| Rwork | 0.199 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.538 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmeas | 0.144 | 0.709 |
| Rpim | 0.071 | 0.410 |
| Number of reflections | 19286 | 885 |
| <I/σ(I)> | 8.9 | 1.7 |
| Completeness [%] | 99.5 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.987 | 0.631 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Bis-Tris pH6.0, 20% PEG3350 |
